Part:BBa_K2958005

Linker for Single Chain Proinsulin and Long Lasting Insulin Analogs

Linker with amino acid sequence GGYLGGGGGGGR used to create single chain insulin and long lasting single chain insulin.

Description:

This linker was used in place of the C chain of Proinsulin to form single-chain insulin. Specifically, Single Chain Insulin and Long Lasting Insulin Analogs (BBa_K2958006 and BBa_K2958007, respectively).. The protein sequence for this linker is GGYLGGGGGGGR. When combined with the A & B chains of proinsulin, the PI of the insulin protein is 5.50 for Single Chain Proinsulin, and 6.46 for Long Lasting insulin, as determined by Serial Cloner. This linker is one of many non-patented linkers listed in the journal article, "Single-Chain Insulins as Receptor Agonists" by Rajpal et al (2009).

References:

Gautam Rajpal, Ming Liu, Yi Zhang, Peter Arvan, Single-Chain Insulins as Receptor Agonists, Molecular Endocrinology, Volume 23, Issue 5, 1 May 2009, Pages 679–688, https://doi.org/10.1210/me.2008-0349

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]